{"id":2427,"date":"2026-01-15T04:05:00","date_gmt":"2026-01-15T04:05:00","guid":{"rendered":"https:\/\/pooja.plumead.com\/?p=2427"},"modified":"2026-02-02T09:38:23","modified_gmt":"2026-02-02T09:38:23","slug":"untargeted-tear-proteomics-in-keratoconus","status":"publish","type":"post","link":"https:\/\/pooja.plumead.com\/index.php\/2026\/01\/15\/untargeted-tear-proteomics-in-keratoconus\/","title":{"rendered":"Untargeted Tear Proteomics in Keratoconus"},"content":{"rendered":"\t\t
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Untargeted Tear Proteomics in Keratoconus<\/h2>

Investigative Ophthalmology & Visual Science (IOVS), 2025<\/strong><\/p>

This study investigated molecular changes in the tear fluid of individuals with keratoconus (KC)<\/strong> using a large South-Asian cohort to identify proteins and pathways that differ across disease stages.<\/p>

Researchers analyzed tear samples from 40 healthy individuals and 107 eyes with varying KC grades<\/strong> using untargeted mass spectrometry (iTRAQ-based LC-MS\/MS) to profile proteins without prior assumptions.<\/p>

Key Findings:<\/strong><\/p>

  • A total of 1104 tear proteins<\/strong> were identified, and 279 were quantified across samples<\/strong>.<\/p><\/li>

  • Thirty-two proteins<\/strong> showed significant changes in expression in KC patients compared with controls, indicating dysregulation of biological processes.<\/p><\/li>

  • Dysregulated proteins were involved in:<\/p>

    • Glycolytic and metabolic pathways<\/strong>, suggesting altered cellular metabolism in KC.<\/p><\/li>

    • Extracellular matrix (ECM) organization<\/strong>, consistent with the corneal structural weakening seen in KC.<\/p><\/li>

    • Reactive oxygen species (ROS) detoxification<\/strong> and inflammatory regulation<\/strong>, highlighting oxidative stress and immune involvement in disease progression.<\/p><\/li><\/ul><\/li>

    • Several proteins such as Cystatin-S, Lacritin, Glutathione Synthetase, and Superoxide Dismutase<\/strong> were validated to show differential expression across KC grades, pointing to potential biomarkers for disease severity.<\/p><\/li><\/ul>

      Conclusions:<\/strong>
      These results reveal novel tear fluid proteins and pathways<\/strong> associated with keratoconus progression, including inflammatory signaling, ECM remodeling, and metabolic alterations. The study\u2019s findings improve understanding of the molecular mechanisms underlying KC and may guide the development of stage-specific biomarkers and therapeutic targets<\/strong>.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

      Untargeted Tear Proteomics in Keratoconus Investigative Ophthalmology & Visual Science (IOVS), 2025 This study investigated molecular changes in the tear fluid of individuals with keratoconus (KC) using a large South-Asian cohort to identify proteins and pathways that differ across disease stages. Researchers analyzed tear samples from 40 healthy individuals and 107 eyes with varying KC<\/p>\n","protected":false},"author":1,"featured_media":1497,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[16],"tags":[],"class_list":["post-2427","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"acf":[],"_links":{"self":[{"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/posts\/2427","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/comments?post=2427"}],"version-history":[{"count":4,"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/posts\/2427\/revisions"}],"predecessor-version":[{"id":2518,"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/posts\/2427\/revisions\/2518"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/media\/1497"}],"wp:attachment":[{"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/media?parent=2427"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/categories?post=2427"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pooja.plumead.com\/index.php\/wp-json\/wp\/v2\/tags?post=2427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}